Cell Culture Media
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Bioworld Linsmaier & Skoog Basal Medium, w/ Sucrose & Agar, pH Adjusted and Buffered, 25 L
Linsmaier & Skoog Basal MediumContains Macronutrients, Micronutrients, and Vitamins as described by Linsmaier & Skoog (1965) with addition of Sucrose and AgarBuffered with MESCytokines and Auxins should be added as needed Provided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture testedWorking Concentration: 42.53 g/LPartially soluble in water Medium may be sold as Murashige & Skoog (MS) Medium with Minimal Organics (MSMO) by some media suppliers
NEOGEN Lactobacilli MRS Broth, 5 kg
Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-broth
NEOGEN Mueller Hinton Agar II, 5 kg
Mueller Hinton Agar II is used in antimicrobial susceptibility testing by the disk diffusion method. This formula conforms to Clinical and Laboratory Standard Institute (CLSI), formerly National Committee for Clinical Laboratory Standards (NCCLS).Mueller Hinton Agar II is based on the formula recommended by Mueller and Hinton for the primary isolation of Neisseria species. Mueller and Hinton selected pea meal extract agar as a simple transparent medium containing heat stable ingredients. During their modification, starch replaced the growth-promoting properties of pea extract, acting as a “protective colloid” against toxic substances. Bauer, Kirby, Sherris and Tuck recommended Mueller Hinton Agar for performing antibiotic susceptibility tests using a single disk of high concentration.For more information visit https://www.neogen.com/solutions/Microbiology/mueller-hinton-agar-2
NEOGEN Lactobacilli MRS Broth, 500 g
Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-broth
Sigma Aldrich Fine Chemicals Biosciences Endothelial cell growth su5MG
Endothelial cell growth supplement (ECGS) comprises acidic fibroblast growth factor (afGf) and basic fGf (bfGf).
American Research Products Inc Mouse acidic fibroblast growth
Mouse acidic fibroblast growth factor aFGF/FGF-1 ELISA Kit from CUSABIO Detection Range 6 25 pg/mL-400 pg/mL Sensitivity 1 56 pg/mL Sample serum plasma tissue homogenates Method Sandwich
Promocell GmbH PromoCell Endothelial Cell Basal Medium 2 500 ml
Endothelial Cell Growth Medium 2 is a low-serum (2% V/V) medium that lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 µg / ml || Heparin | 22.5 μg / ml || Hydrocortisone | 0.2 µg / ml |For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Promocell GmbH PromoCell Cytokine Mix E HPC Expansion Medium XF 5 ml, (sufficient for 500 ml Medium)
The PromoCell Hematopoietic Progenitor Cell (HPC) Expansion Medium XF provides a highly optimized, serum-free and xeno-free culture system allowing for superior expansion performance of human hematopoietic progenitor cells, e.g. CD34+ and CD133+ cells. As a result the expanded cell population is enriched with functional progenitor cells capable of CFC-formation in appropriate in vitro assays.Cytokine Mix E is a ready-to-use mixture of growth factors optionally available for the HPC Expansion Medium XF. The serum-free and xeno-free formula contains recombinant human TPO, SCF, FLT-3 ligand and IL-3.With traditional serum-containing media, the majority of HPC lose their functionality during the expansion process. Our optimized serum-free and xeno-free formulation allows for an expanded cell population that is enriched with functional progenitor cells capable of CFC-formation in appropriate in vitro assays.
Genscript Corporation eBlot L2 Transfer Basic Kit m
eBlot L2 Transfer Basic Kit Midi is a concentrated rapid membrane transfer kit used with the eBlot L2 Protein Transfer Device (Cat No L00981) It supports the transfer of 30 midi gels or 60 mini gels using the standard protein transfer method
NEOGEN Clostridium Difficile Agar Base, 5 kg
Clostridium Difficile Agar Base (Brazier’s) is used with blood, Cycloserine, and Cefoxitin for the isolation of Clostridium difficile. Clostridium Difficile is not intended for use in the diagnosis of disease or other conditions in humans.Brazier’s CCEY agar is the formulation currently used by the Anaerobe Reference Unit for the isolation of C. difficile, resulting from work initiated by Ken Phillips and Paul Levett, and completed by Jon Brazier. Based upon the market leading anaerobe medium, Fastidious Anaerobe Agar, it incorporates additional ingredients to improve the isolation and differentiation of C. difficile from samples.Cholic acid is present to promote spore germination following alcohol shock treatment, and p-hydroxyphenylacetic acid to enhance the production of p-cresol, a distinctive metabolite of C. difficile.For more information visit https://www.neogen.com/solutions/Microbiology/clostridium-difficile-agar-base
Sigma Aldrich Fine Chemicals Biosciences Endothelial Cell Growth Me
Endothelial Cell Basal Medium 2 (supplements sold separately)
ATHENA ES APF LB MILLER 500GR
Athena offers three variations of the basic nutrient-rich LB Broth media (Miller, Lennox, and Luria) as well as their APF Certified™ equivalents made using Athena’s proprietary blended plant protein hydrolysate: Atholate™. The formulations each provide an abundance of peptides and peptones, vitamins and trace elements, and differ only in sodium chloride content and whether they were made with casein hydrolysate or Atholate™. The differences allow the researcher to select which formula will provide the ideal osmotic conditions for their particular bacterial strain and desired culture conditions. Each LB Broth and APF LB Broth can be custom ordered in bulk.
Bioworld BG-A2 Medium, 25 L
For selection and recovery of transgenic plants (eg., Bermuda grass) infected with Agrobacterium tumefaciens.Specification:Appearance: cream to yellow powderSolubility: Partially soluble in waterRecommenced use: 12.4g/LContains: Murashige & Skoog medium supplemented with 4.5uM Kinetin, 0.2uM 2,4-D, 0.8% agar.Add: Aseptically add filter-sterilized hygromycin (75mg/L), silver nitrate (1.7mg/L), cefotaxime (250,g/L) and sucrose (2%) to the pre-sterilized BG-A2 medium or as required.